MMG-203
MAMMLIAN CELL CULTURE IN
BIOMEDIAL RESEARCH

LABORATORY INTRODUCTION

    THE "LABORATORY"
I.  Objective: To construct the laboratory environment for the course:  In essence each laminar flow hood (there are four of them) and its environs will constitute a "laboratory" and each group of four to five students will constitute its "occupants". Students will be divided into four (the number of "laboratories") groups of either four or five depending upon the number of students registered for the course. Each group of four or five will then constitute one of the "laboratories".

II.  Procedure: Each "laboratory" will be responsible for supplying the needs of the lab. For example, each lab will make its own media for use in growing the cell cultures, stocking up on the various size flasks which will be used, obtaining the cells for culturing, planting the cells for each of the experiments to be run, etc. We will, of course, have discussed, in detail, each experiment to be run prior to actually doing the experiment. However, the "carrying out" of the experiment, maintaining notebooks and presenting the data, will be the responsibility of the "lab". Therefore, it is imperative that each "lab" comes prepared and is completely clear what they have to do and what they need to do the experiment. You must read ahead to see what amounts of cells you need to plant THIS WEEK to be used in the subsequent experiment. The Teaching Assistants will be there as support people. Although we will have pre-ordered sufficient numbers of the various flasks and other supplies (pipets, dishes, bottles, etc.), it will be the responsibility of each "lab" inform a TA of your needs so that the order can be filled.

III.  You should become familiar with the available catalogs, from which the particular item(s) the lab needs may be purchased.

IV.  Starting Materials For Each Laboratory: Cells, culture media, culture flasks, pipets (0.5, 1.0, 2.0, 5.0, 10.0 and 25.0 ml), hemocytometer, pour-off flasks, pipeters, sterile media bottles, 15 and 50 ml centrifuge tubes, inverted microscope, safety disposal containers, desk-top centrifuges plus other materials as needed.

V.  Discussion: Discuss the setting up of a cell culture laboratory

VI.  Laboratory Notebook

     
     
     
     
     

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