MMG-203
MAMMLIAN CELL CULTURE IN
BIOMEDIAL RESEARCH

LISTING OF COURSE LECTURE & LABS

COURSE LIST OF LECTURES
  1. Introduction to the course and laboratory
  2. What is cell culturing all about? What are cell cultures?  How are they obtained? Culturing and counting of cells
  3. Cell culture lecture cont'd.
  4. Theory of freeze storage of cells. "Basic Cell Culture I"
  5. Cell culture media. 
  6. "Basic Cell Culture II" repeat + Media cont'd..
  7. Discussion of Basic Cell Culture I Laboratory
  8. Serum-free growth of cells
  9. Lab meeting to discuss all growth experiments & discuss setup of "Serum-Free Exp't."
  10. Soft agar growth   - theory & practice
  11. Karyology of cells in culture - How-to and Why-to
  12. Mycoplasmas, The Scurge of Cell Cultures
  13. Long-Term Culture of Cells + Lab meeting to discuss Mycoplasma Experiment data.
  14. Guest speaker -
  15. University Holiday for Town Meeting
  16. Lab meeting to discuss Serum-free, Soft Agar & Karyology experimental data.
  17. Guest speaker -
  18. Guest speaker -
  19. Spring Break-two lecture sessions
  20. Molecular Biology of cultured cells-lectures
COURSE  LIST OF LAB SESSIONS
  1. Tour of the lab/Set up lab groups/Check equipment list
  2. Lab.- Make your first medium /check for contamination
  3. Lab.- Set up for "Basic Cell Culture-I" experiment (this experiment runs for 72 hrs.)
  4. Lab.-  "Basic Cell Culture II-repeat" experiment (runs for 72 hrs.)/"Freeze cells"
  5. Lab.- "Unfreeze" test cells
  6. Lab - Receive your own test cells and set up for "Basic Cell Culture-III" experiment with your own cells-(runs for 72 hrs.)
  7. Lab - Discussion of Growth experiments and format of "Serum-Free" experiment.
  8. Lab - Run "Serum-Free" experiment-runs for 144 hrs.)
  9. Lab - Run  "Soft Agar" experiment .
  10. Lab - Stain and count clone plates from "Serum-Free" experiment
  11. Lab - Plant cells for "Karyology" and "Mycoplasma" experiments & Count soft agar plates
  12. Lab -  Do chromosome spreads and observe.
  13. Lab - Set up  "Mycoplasma" experiment
  14. Lab - Stain for mycoplasma and evaluate.
  15. Spring Break - over two laboratory periods.
  16. Lab -Set up for Molecular Biology experiments. .
  17. Lab exercises for Molecular Biology .

    18.  

 

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