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| Bioinformatics Core | Mentoring Core | Microarray Facility | ||
| Microarray Outreach Page | ||||
Microarray OutreachAbout usThe Microarray Outreach project of the Vermont Genetics Network was made possible through a grant supplement to the VGN grant funded by the NCRR. A Microarray Outreach Team was assembled in early fall of 2003. The team developed a hands-on undergraduate microarray laboratory module using the Affymetrix microarray platform to take to undergraduate schools in Vermont. The beta test of the module was run at the University of Vermont in the fall of 2003 and delivered to St. Michael's College, Middlebury College, and Johnson State College in the spring semester of 2004. We currently have scheduled Green Mountain College and Norwich University for the fall 2005 and Castleton State College in the spring semester 2006. Team Members:
The goal of the VGN Microarray Outreach project is to expose undergraduates in the state of Vermont to microarray technology using hands-on laboratory experiences. We provide all teaching materials, laboratory materials, and laboratory equipment for schools within the state to run the module. All materials become the property of the recipient institution upon completion of the microarray laboratory module. We hope to increase the skills of undergraduate science students within the state and hope that these additional skills help them in their future career development. OVERVIEW:In this module, students will learn how gene expression in yeast is changed after exposure of yeast to dimethyl sulfoxide (DMSO), a common environmental contaminant and solvent. Control yeast are exposed to water and "treated" yeast are exposed to 10% DMSO for 2 hours. RNA is then harvested and prepared for use on Affymetrix® yeast microarray chips. Prepared cRNA is taken back to the UVM Microarray Core Facility and hybridized with the yeast chips. The data are processed using the most recent probeset signal intensity algorithms including RMA and GCRMA, the primary software utilized in visualizing gene expression differences is Spotfire®. Students are then taken through basic microarray data analysis procedures to generate lists of genes which are up or down regulated upon treatment with DMSO. MATERIALS:Laboratory Manual
Microarray Useful Links:
Data from Green Mountain College Fall 2005: Data from Norwich University Fall 2005:
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