Go to the
"Gene" data base. Type “ferrochelatase” into the search box and click
on the “Go” button. Under the “Genomic regions, transcripts and products”
section find the link to “NC000018” and click on it, then select “GenBank”.
Make sure that the accession number at the top of the GenBank record matches
the accession number given in the "Gene" database record.
Copy this page and
paste into a Microsoft Word document. Type the answers in bold after each question. When you have answered each of
the questions, print the document and hand it in to your
Instructor.
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EXERCISE
2: Genbank. NAME:
__________________________________
1.
What is the gi number
of this record?
2.
What kind of sequence
is this?
3.
How many bases are
there in the sequence?
4.
What is the product of
this gene?
5.
In one precisely
worded, carefully organized sentence, describe the function of the gene
product.
6.
What is the first base
of the coding sequence?
7.
What is the second base
of the third codon?
8.
What is the 5th codon?
9.
What is the 6th amino
acid?
10. There are three stop codons. Which one occurs in the
gene for delta globin?
11. Where does the poly-A region begin?
12. How many bases are present in this gene?
13. How many bases are present in the coding sequence of
this gene?
14. How many amino acids are present in the coding
sequence of this gene?
15. The gene begins with base 1, but the CDS does not.
EXPLAIN!
16. What is the number of the second base in the stop
signal?
17. How long is the 3' UTR?
18. How many introns are present in this gene?
19. What are the first 5 bases in the gene?
20. What is the number of the second base in the start
signal?
21. What are the first 5 bases of the coding sequence?
22. How long is the 5' UTR?
23. What is the start signal for the FECH gene?
24. How many exons are present in this gene?
25. Which of the three stop signals is used in the FECH
gene?
26. How long is the gene?
27. How long is exon-1?
28. Which is the 14th codon in the second exon of FECH?
29. What amino acid does this codon code for?
30. How long is exon-3?
31. Which is the 4th codon in the 3rd exon of FECH?
32. What amino acid does this codon code for?
33. Which is the 9th codon in the first exon of FECH?
34. What amino acid does this codon code for?
35. How long is exon-2?
36. What are bases 11-17 after the splice signal of
intron #1?
37. What are the last 5 bases before the splice signal at
the end of intron #1?
38. What are the last 5 bases before the splice signal at
the end of intron #1?
39. What are bases 8-13 after the splice signal of intron
#1?
40. What are bases 10-15 after the splice signal of
intron #1?
41. What are the last 5 bases before the splice signal at
the end of intron #1?
42. What are the last 5 bases in the gene?
43. What are the last 5 bases of the coding sequence?
44. Which of the three stop codons is used in the FECH
gene?
45. How long is the 3' UTR?