DNA Fingerprinting
Why so much DNA?
Genome Sizes
“junk” DNA
Mini-Satellites or VNTRs
The Locus of a Gene
Loci of common VNTRs
Inheritance of VNTR Genotypes
Determining the Genotype at a VNTR Locus
DNA Replication
In vitro DNA Replication
PCR
Amplifying a VNTR by PCR
DNA in forensic samples
VNTR and electrophoresis
In standard forensic practice, there are several VNTRs which are used. This increases the ability of forensic DNA analyses to distinguish between suspects.
PPT Slide
Evidence from a murder case: amplification of VNTR DNA by PCR
Evidence from a rape case
Beyond a Reasonable Doubt
Product Rule
What is the probability of a genetic match in samples “B” and “F” with 3 VNTR loci?
Suppose that sample “F” is from the crime scene and sample “B” is from a suspect
Ugly Facts
There are many human populations. Allele frequencies vary between them.
VNTR loci have many alleles
Allele frequencies vary between sub-populations of ethnic populations
Why are sub-populations important?
CODIS
American sub-populations
13 CODIS STRs (VNTRs)
the end
Email: barnes@clarion.edu
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