NAME ____________________________________________

1. Using human beta globin do a Blastp in search in the Biology Workbench. How are whales, seals, sea lions and dolphins, rats, horses, rabbits, sheep and cows related to each other (or any other 9 mammalian sequence as compared to human) ?
a. Paste your BOXSHADE output here:
 
b. Paste your unrooted tree here:
 
c. Paste your rooted tree here:
 
 
 
 
2. Serum albumin is the most common protein in blood serum, and is a key datum in routine blood tests. It is synthesized in the liver, so decreased levels (the normal range in humans is 3.4 – 5.4 g/dL)
 are an indication of liver disease. It is essential in maintaining the osmotic pressure of the blood, so that fluid does not accumulate in the tissues. Do a TBLASTN using human serum albumin
 (NP_000468; gi4502027) as the query sequence. Produce a rooted and unrooted tree for vertebrates from a multiple alignment of serum albumin sequences (Try to pick similar species as above).
 
a. Paste your BOXSHADE output here:
 
b. Paste your unrooted tree here:
 
c. Paste your rooted tree here:
 
d. Are the evolutionary relationships based on serum albumin sequences the same as the evolutionary relationships based on myoglobin sequences? EXPLAIN (approximately 5 precisely worded, concise and carefully organized sentences).