BLASTP Search for Homologs to Human Myoglobin     BLASTP searches in designated protein databases such as PIR or SWISSPROT for matches to the imported sequence (either DNA or protein).  The user is permitted to select one or more databases to compare. Since human myoglobin was selected, BLASTP will find all of the animals, plants, bacteria and viruses, that have similar sequences. Since myoglobin is an oxygen transport protein, most often found in muscles, it is unlikely that there will be any hits from  plants, fungi, bacteria or viruses.  Click on the thumbnail to see full-size! The "Protein Tools" page should now show that the sequence for human myoglobin has been imported.  Click on the check box opposite "human myoglobin". Scroll down the tools and select BLASTP. CLICK ON THE "RUN" button. Click on the thumbnail to see full-size! The "BLASTP" page provides a scroll box from which various databases may be chosen for BLASTP to search. Scroll down and select the "Swiss Prot" data base. Scroll to the bottom of the page, accepting all the default values. Click the "Submit" button. Click on the thumbnail to see full-size! Results are returned as shown in the thumbnail to the left (a large red flag alerts that the default hit limit of 100 has been exceeded). Click on the thumbnail to see full-size! The hit list is presented in descending order of bit score and E-value. This provides a rough estimate of the sequences which are the closest match to human myoglobin. The descriptions of each hit are incomplete, and may be impossible to decipher. Click on the checkbox for the record of myoglobin from Hylobates agilis. Then click on the "Show Records" button. The record (thumbnail to the left) shows that this sequence was obtained from the skeletal muscle of the Agile Gibbon. Select at least 10 sequences from the following list by clicking in the checkboxes.  NOTE:  It is not necessary to select one of the human hits because human myoglobin is the query sequence.   chimpanzee mountain gorilla orangutan olive baboon egyptian fruit bat pig eurasian beaver eurasian river otter black-capped capuchin aardvark opossum south american pika amazon dolphin california sea lion horse plains zebra rabbit harbor porpoise pilot whale atlantic dolphin killer whale humpback whale sperm whale dog harbor seal indian elephant sheep red deer cow rat mouse chicken alligator Scroll to the top of the page and click on the "Import Sequences" button. Click on the thumbnail to see full-size! Biology Workbench returns to the "Protein Tools" page. As shown in the thumbnail all of the selected sequences have been imported.

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