January 14 - Tuesday
|
Introduction to the course and the cell culture
laboratory
|
Chapters 3-6
|
16 - Thursday
|
What is cell culturing all about?
What are cell cultures? How are they obtained? Culturing and
counting of cells
|
Chapters 1-2, 11-13
|
January 20 - Monday
|
MARTIN LUTHER KING HOLIDAY
|
|
January 21 - Tuesday
|
Cell culturing, continued
|
Chapter 15
|
23
- Thursday
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Theory of freeze storage of cells.
|
Chapter 19
|
January 28 - Tuesday
|
Cell culture media
|
Chapters 8-10
|
30
- Thursday
|
Media, continued. Discuss rationale
for next week's experiment: Basic Cell Culture III
|
|
February 4 - Tuesday
|
Discussion of Basic Cell Culture I Laboratory
|
|
6 - Thursday
|
Serum-Free media; Theory and Practice
of using soft agar Growth
|
Ch. 9, & 17, pgs.277-8
|
February 11 - Tuesday
|
LAB MEETING: Discussion of ALL growth
experiments; Discussion of the setup for Serum-Free experiment.
|
|
13 - Thursday
|
Karyology of cells in culture: How-to and Why-to;
Mycoplasmas: The "crabgrass" of cell cultures!
|
Chapters 15 & 18
|
February 17 - Monday
|
PRESIDENTS' DAY HOLIDAY
|
|
February 18 - Tuesday
|
Long-term culture of cells and their scientific
use
|
|
20 - Thursday
|
Guest Lecturer
|
|
February 25 - Tuesday
|
Guest Lecturer
|
|
27 - Thursday
|
Guest Lecturer
|
|
March 4 - Tuesday
|
TOWN MEETING DAY HOLIDAY
|
|
6
- Thursday
|
LAB MEETING: Discussion of Serum-Free, Soft
Agar and Karyology data.
|
|
March 11 - Tuesday
|
Guest Lecturer
|
|
13 - Thursday
|
Guest Lecturer
|
|
Week of March 17-21
|
SPRING BREAK --- ENJOY!!!
|
|
March 25 - Tuesday
|
Overview of transfection experiments
|
|
27 - Thursday
|
Transfection and myoD
|
|
April 1 - Tuesday
|
Indirect immunofluorescence
|
|
3 - Thursday
|
LAB MEETING: Open discussion of results so
far.
|
|
April 8 - Tuesday
|
First Lab Group Presentation
|
|
10 - Thursday
|
Protein extracts
|
|
April 15 - Tuesday
|
SDS PAGE electrophoresis
|
|
17 - Thursday
|
Western transfer
|
|
April 22 - Tuesday
|
Western detection
|
|
24 - Thursday
|
Second Lab Group Presentation
|
|
April 29 - Tuesday
|
Last two Lab Group Presentations + Any
leftover lab discusssions
|
|
April 30 - Wednesday
|
LAST DAY OF CLASSES
|
|